求06版FDA沙门氏菌检测（英文原版）
发布网友
发布时间：2022-04-20 09:38
共3个回答
热心网友
时间：2023-10-05 19:02
FDA上有这样的（http://www.cfsan.fda.gov.sixxs.org/~frf/forum06/L-10.htm， 可惜不能下载全文）：
Evaluation of VIDAS® Immuno-Concentration Salmonella (ICS) and VIDAS® Salmonella Immunoassay (SLM) for rapid detection of Salmonella spp. in selected foods
R. Zapata, C. N. Wendakoon, C. Carrillo, P. Browning, W. M. Fedio, Food Safety Laboratory, New Mexico State University
Background: Detection of Salmonella spp. in foods is based on conventional enrichment and isolation on selective media. AOAC International has approved a rapid automated method using the VIDAS® Immuno-Concentration Salmonella (ICS), which captures Salmonella cells for subsequent detection by the VIDAS immunofluorescent (SLM) assay. The reliability of the VIDAS ICS/SLM method was compared with the FDA-BAM procere for Salmonella detection in selected foods artificially contaminated with Salmonella Typhimurium.
Methods: Whole milk, powdered infant formula, almonds, strawberries, cilantro, parsley, bok choy, lettuce, cantaloupe andmung bean sprouts were artificially contaminated with Salmonella Typhimurium (ATCC 14028) at low (1-5 CFU/25g) and high (10-50 CFU/g) levels. At each level, 20 samples were analyzed for Salmonella spp. by the two methods. For each food, five uninoculated control samples were also tested. McNemar's χ2 test was used to determine significant differences between the two methods at each inoculum level.
Results: No statistical differences (p ≥ 0.05) were found between the two methods except for mung bean sprouts inoculated at the low level. The VIDAS method failed to detect 15 inoculated mung bean samples shown to contain Salmonella spp. by the BAM procere (100% false negative rate). Parsley, bok choy, cilantro and cantaloupe inoculated at low levels also gave false negative results with the VIDAS method.
Conclusion: The VIDAS ICS/SLM method was shown to be convenient and considerably faster than the BAM procere for Salmonella detection. However, for foods with a high background microflora, false negative results were seen, particularily at low levels of contamination.
有CDC的2006年年度报道。 http://www.cdc.gov.sixxs.org/ncidod/dbmd/phlisdata/salmtab/2006/SalmonellaIntroction2006.pdf
热心网友
时间：2023-10-05 19:02
不知道你是说有这么一本书还是FDA沙门氏菌检测的资料
自己去FDA官网看看吧，有的话就应该有。
http://google2.fda.gov/search?access=p&entqr=0&getfields=*&output=xml_no_dtd&sort=date%3AD%3AL%3Ad1&ie=UTF-8&lr=&client=FDA&q=2006&q=salmonella&ud=1&y=18&oe=UTF-8&proxystylesheet=FDA&ip=61.175.193.50&x=17&start=10
热心网友
时间：2023-10-05 19:03
Why??
